1DLO
HUMAN IMMUNODEFICIENCY VIRUS TYPE 1
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Detector technology | IMAGE PLATE AREA DETECTOR |
Collection date | 1994-10 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 235.500, 70.300, 93.300 |
Unit cell angles | 90.00, 106.10, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.700 |
R-factor | 0.249 |
Rwork | 0.249 |
R-free | 0.33600 |
RMSD bond length | 0.010 * |
RMSD bond angle | 2.000 |
Data reduction software | DENZO |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.700 * |
Rmerge | 0.055 |
Total number of observations | 142770 * |
Number of reflections | 41049 |
Completeness [%] | 99.5 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 * | Kohlstaedt, L.A., (1992) Science, 256, 1783. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | RT | 6-8 (mg/ml) | |
10 | 1 | reservoir | beta-octylglucoside | 0.2 (%(w/v)) | |
11 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
12 | 1 | reservoir | PEG8000 | 14 (%(w/v)) | |
13 | 1 | reservoir | sodium azide | 0.02 (%) | |
2 | 1 | drop | Bis-Tris propane | 25 (mM) | |
3 | 1 | drop | 50 (mM) | ||
4 | 1 | drop | beta-octylglucoside | 0.1 (%(w/v)) | |
5 | 1 | drop | glycerol | 5 (%(v/v)) | |
6 | 1 | drop | PEG8000 | 7 (%(w/v)) | |
7 | 1 | drop | sodium azide | 0.01 (%) | |
8 | 1 | reservoir | Bis-Tris propane | 50 (mM) | |
9 | 1 | reservoir | 100 (mM) |