1DLJ
THE FIRST STRUCTURE OF UDP-GLUCOSE DEHYDROGENASE (UDPGDH) REVEALS THE CATALYTIC RESIDUES NECESSARY FOR THE TWO-FOLD OXIDATION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-02-20 |
Detector | BRANDEIS - B4 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 106.961, 106.961, 81.744 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.890 - 1.800 |
R-factor | 0.179 |
Rwork | 0.179 |
R-free | 0.21300 |
RMSD bond length | 0.011 |
RMSD bond angle | 23.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.900 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.045 | 0.510 |
Total number of observations | 176474 * | |
Number of reflections | 38487 | |
<I/σ(I)> | 28.8 | |
Completeness [%] | 87.3 * | 42.4 |
Redundancy | 4.6 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | ambient temperature * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 2-2.1 (M) | |
2 | 1 | reservoir | glycerol | 6-8 (%) | |
3 | 1 | reservoir | Tris-HCl | 100 (mM) |