1DLI

THE FIRST STRUCTURE OF UDP-GLUCOSE DEHYDROGENASE (UDPGDH) REVEALS THE CATALYTIC RESIDUES NECESSARY FOR THE TWO-FOLD OXIDATION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-11-19
Detector	MARRESEARCH
Spacegroup name	P 4 21 2
Unit cell lengths	106.601, 106.601, 81.592
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.000 * - 2.300 *
R-factor	0.186
Rwork	0.186
R-free	0.25900
RMSD bond length	0.011
RMSD bond angle	23.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.300 *	2.340
High resolution limit [Å]	2.300 *	2.300
Rmerge	0.082	0.377
Total number of observations	119755 *
Number of reflections	20008
<I/σ(I)>	18.5
Completeness [%]	95.1 *	76.2
Redundancy	6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	ambient temperature *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.6-1.7 (M)
2	1	reservoir	glycerol	6-8 (%)
3	1	reservoir	Tris-HCl	100 (mM)