1DLH

CRYSTAL STRUCTURE OF THE HUMAN CLASS II MHC PROTEIN HLA-DR1 COMPLEXED WITH AN INFLUENZA VIRUS PEPTIDE

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	94.600, 94.600, 247.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.800
R-factor	0.205
Rwork	0.205
R-free	0.30400
RMSD bond length	0.018
RMSD bond angle	2.130
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.750 *	2.750 *
Rmerge	0.067 *	0.231 *
Total number of observations	88058 *
Number of reflections	29857 *
Completeness [%]	94.5 *	89.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			referred to 'Stern, L.J.', (1992) Cell, 68, 465-477 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	14-17 (%)
2	1	reservoir	glycine	100 (mM)