1DLG
CRYSTAL STRUCTURE OF THE C115S ENTEROBACTER CLOACAE MURA IN THE UN-LIGANDED STATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-20 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.685, 155.532, 83.820 |
| Unit cell angles | 90.00, 91.82, 90.00 |
Refinement procedure
| Resolution | 14.970 - 1.900 |
| R-factor | 0.173 * |
| Rwork | 0.173 |
| R-free | 0.19700 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.970 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.033 | 0.119 |
| Total number of observations | 703604 * | |
| Number of reflections | 86085 | |
| <I/σ(I)> | 35.8 | |
| Completeness [%] | 99.2 | 91.9 |
| Redundancy | 7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 * | 292 | 0.4-0.8 M sodium/potassium phosphate including 20-40 mM cyclohexylammonium phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 19K, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium potassium phosphate | 325 (mM) | pH6.9 |
| 2 | 1 | drop | dithiothreitol | 1 (mM) | |
| 3 | 1 | drop | protein | 40 (mg/ml) | |
| 4 | 1 | drop | cyclohexylammonium phosphate | 20 (mM) | |
| 5 | 1 | reservoir | sodium potassium phospahte | 1 (M) | pH6.5 |
