1DKM
CRYSTAL STRUCTURE OF ESCHERICHIA COLI PHYTASE AT PH 6.6 WITH HG2+ CATION ACTING AS AN INTERMOLECULAR BRIDGE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-01-23 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.026, 74.543, 89.359 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.250 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.26600 |
RMSD bond length | 0.006 |
RMSD bond angle | 22.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.030 | 0.085 |
Number of reflections | 42339 * | |
<I/σ(I)> | 17.75 | |
Completeness [%] | 96.8 | 98.6 |
Redundancy | 1.95 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.6 | 298 | ETHYLENE GLYCOL, GLYCEROL, 2-MORPHOLINOPROPANESULFONIC ACID, MERCURIC CHLORIDE, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 72 (mg/ml) | |
2 | 1 | drop | 2.5 (mM) | ||
3 | 1 | reservoir | ethylene glycol | 28 (%) | |
4 | 1 | reservoir | glycerol | 18 (%) | |
5 | 1 | reservoir | Mops | 33 (mM) |