1DKL
CRYSTAL STRUCTURE OF ESCHERICHIA COLI PHYTASE AT PH 4.5 (NO LIGAND BOUND)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.802, 72.126, 82.434 |
| Unit cell angles | 90.00, 92.04, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.157 |
| Rwork | 0.157 |
| R-free | 0.20200 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.085 | 0.231 |
| Number of reflections | 37076 | |
| <I/σ(I)> | 12.46 | |
| Completeness [%] | 95.0 | 97 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.6 * | 273 | SODIUM ACETATE, pH 4.5, SMALL TUBES, temperature 273K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 72 (mg/ml) | |
| 2 | 1 | drop | 2.5 (mM) | ||
| 3 | 1 | reservoir | ethylene glycol | 28 (%) | |
| 4 | 1 | reservoir | glycerol | 18 (%) | |
| 5 | 1 | reservoir | Mops | 33 (mM) |
