1DKH

CRYSTAL STRUCTURE OF THE HEMOPHORE HASA, PH 6.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-09-29
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	111.650, 111.650, 52.790
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.000 - 3.200
R-factor	0.23
Rwork	0.228
R-free	0.28800
RMSD bond length	0.009
RMSD bond angle	1.460
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.074	0.291
Number of reflections	6229
<I/σ(I)>	7.4
Completeness [%]	100.0	99.7
Redundancy	3.9	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	20 *	Arnoux, P., (1999) Nat.Struct.Biol., 6, 516. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	cacodylate	100 (mM)
3	1	reservoir	zinc acetate dihydrate	140 (mM)
4	1	reservoir	glycerol	2 (%)
5	1	reservoir	PEG8000	9-11 (%)