1DKH
CRYSTAL STRUCTURE OF THE HEMOPHORE HASA, PH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-29 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 111.650, 111.650, 52.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 3.200 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.28800 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.460 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.074 | 0.291 |
| Number of reflections | 6229 | |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 20 * | Arnoux, P., (1999) Nat.Struct.Biol., 6, 516. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | cacodylate | 100 (mM) | |
| 3 | 1 | reservoir | zinc acetate dihydrate | 140 (mM) | |
| 4 | 1 | reservoir | glycerol | 2 (%) | |
| 5 | 1 | reservoir | PEG8000 | 9-11 (%) |
