1DKG
CRYSTAL STRUCTURE OF THE NUCLEOTIDE EXCHANGE FACTOR GRPE BOUND TO THE ATPASE DOMAIN OF THE MOLECULAR CHAPERONE DNAK
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1996-10 |
Spacegroup name | P 41 |
Unit cell lengths | 149.430, 149.430, 49.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.31700 |
Structure solution method | MIR |
RMSD bond length | 0.012 * |
RMSD bond angle | 1.580 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.093 * | 0.179 * |
Total number of observations | 754420 * | |
Number of reflections | 26024 | |
<I/σ(I)> | 18 | 7 |
Completeness [%] | 95.7 | 86.4 |
Redundancy | 29 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.6 | 4 * | pH 4.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 40 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 3.5 (%) | |
3 | 1 | reservoir | ethylene glycol | 15 (%) | |
4 | 1 | reservoir | sodium acetate | 50 (mM) | |
5 | 1 | reservoir | lithium sulfate | 190 (mM) | |
6 | 1 | reservoir | dithiothreitol | 5-10 (mM) | |
7 | 1 | reservoir | (D,L)-methionine | 10 (mM) |