1DK7
CRYSTAL STRUCTURE OF AN ISOLATED APICAL DOMAIN OF GROEL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 1996-11-29 |
| Detector | BRANDEIS |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 84.859, 84.859, 77.068 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.150 - 2.020 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.24100 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.064 | 0.327 |
| Number of reflections | 18450 | |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 83.6 | 96.9 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 | 292.3 | PEG4K, LI2SO4, TRISHCL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 19.3K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | apocal domain | 1:1(v/v) ratio of 10 mg/ml of the apical domain to well solution | |
| 2 | 1 | reservoir | Tris/Cl | 100 (mM) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | PEG4000 | 30 (%) |
