1DJY
PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C-DELTA1 FROM RAT COMPLEXED WITH INOSITOL-2,4,5-TRISPHOSPHATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-03-28 |
| Detector | MARRESEARCH |
| Spacegroup name | F 41 3 2 |
| Unit cell lengths | 397.130, 397.130, 397.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.2 * |
| Rwork | 0.210 |
| R-free | 0.26000 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 1ISD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 18.476 * |
| Data reduction software | MOSFLM |
| Phasing software | CCP4 |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 * | |
| High resolution limit [Å] | 2.800 * | |
| Rmerge | 0.094 | 0.364 * |
| Total number of observations | 396081 * | |
| Number of reflections | 59742 | |
| Completeness [%] | 90.9 | 91.4 * |
| Redundancy | 6.6 | 4.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 12 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | CHAPSO | 0.1 (%(w/v)) | |
| 3 | 1 | drop | ammonium phosphate | 2.0 (M) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | ammonium phosphate | 1.45 (M) | |
| 6 | 1 | reservoir | dithiothreitol | 1 (mM) |
