1DJX
PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C-DELTA1 FROM RAT COMPLEXED WITH INOSITOL-1,4,5-TRISPHOSPHATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11-04 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | F 41 3 2 |
| Unit cell lengths | 397.540, 397.540, 397.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.217 * |
| Rwork | 0.220 |
| R-free | 0.27000 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.305 |
| Data reduction software | MOSFLM |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.000 * | |
| High resolution limit [Å] | 2.300 * | |
| Rmerge | 0.062 | 0.370 * |
| Total number of observations | 755288 * | |
| Number of reflections | 113379 | |
| Completeness [%] | 96.4 | 94.9 * |
| Redundancy | 6.7 | 5.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 12 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | CHAPSO | 0.1 (%(w/v)) | |
| 3 | 1 | drop | ammonium phosphate | 2.0 (M) | |
| 4 | 1 | drop | dithiothreitol | 1 | |
| 5 | 1 | reservoir | ammonium phosphate | 1.45 (M) | |
| 6 | 1 | reservoir | dithiothreitol | 1 (mM) |
