1DJH

PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C-DELTA1 FROM RAT COMPLEXED WITH BARIUM

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-02-12
Detector	MARRESEARCH
Spacegroup name	F 41 3 2
Unit cell lengths	398.250, 398.250, 398.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.21 *
Rwork	0.210
R-free	0.27000
RMSD bond length	0.015
RMSD bond angle	1.430
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000 *
High resolution limit [Å]	2.500 *
Rmerge	0.067	0.271 *
Total number of observations	384574 *
Number of reflections	92551
Completeness [%]	99.7	99.9 *
Redundancy	4.1	4.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	12 *	Essen, L.O., (1996) Nature, 380, 595. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium phosphate	1.4 (M)
2	1	drop	protein	5 (mg/ml)
3	1	drop	Tris-HCl	5 (mM)
4	1	drop		50 (mM)
5	1	drop	EGTA	0.25 (mM)
6	1	drop	DTT	0.5 (mM)
7	1	drop	sodium azide	0.005 (%(w/v))
8	1	drop	CHAPSO	0.05 (%(w/v))
9	1	drop	ammonium phosphate	0.7 (M)