1DJG

PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C-DELTA1 FROM RAT COMPLEXED WITH LANTHANUM

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Detector technology	IMAGE PLATE
Collection date	1995-11-04
Detector	MARRESEARCH
Spacegroup name	F 41 3 2
Unit cell lengths	397.920, 397.920, 397.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.600
Rwork	0.210
R-free	0.27000
RMSD bond length	0.016
RMSD bond angle	1.410
Data reduction software	MOSFLM
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.000 *
High resolution limit [Å]	2.600 *
Rmerge	0.042	0.170 *
Total number of observations	384052 *
Number of reflections	81095
Completeness [%]	98.5	90.2 *
Redundancy	4.8	2.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	12 *	Essen, L.O., (1996) Nature, 380, 595. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium phosphate	1.4 (M)
2	1	drop	protein	5 (mg/ml)
3	1	drop	Tris-HCl	5 (mM)
4	1	drop		50 (mM)
5	1	drop	EGTA	0.25 (mM)
6	1	drop	DTT	0.5 (mM)
7	1	drop	sodium azide	0.005 (%(w/v))
8	1	drop	CHAPSO	0.05 (%(w/v))
9	1	drop	ammonium phosphate	0.7 (M)