1DJA

STRUCTURE OF BETA-LACTAMASE PRECURSOR, K73H MUTANT, AT 298K

Experimental procedure

Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1995-11-06
Detector	SIEMENS
Spacegroup name	I 2 2 2
Unit cell lengths	53.900, 93.600, 139.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.900
R-factor	0.168
Rwork	0.168
RMSD bond length	0.017
RMSD bond angle	1.800
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.7)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.900 *
Rmerge	0.067	0.218 *
Total number of observations	61878 *
Number of reflections	21589	1971 *
Completeness [%]	79.0	44 *
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *		drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	10 (mg/ml)
2	1	reservoir	ammonium sulfate	76-78 (%sat)
3	1	reservoir	PEG1000	0.4 (%(w/v))
4	1	reservoir		0.3 (M)
5	1	reservoir		0.1 (M)