1DJ0
THE CRYSTAL STRUCTURE OF E. COLI PSEUDOURIDINE SYNTHASE I AT 1.5 ANGSTROM RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-08-25 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.080, 37.400, 103.480 |
| Unit cell angles | 90.00, 97.68, 90.00 |
Refinement procedure
| Resolution | 38.000 - 1.500 |
| R-factor | 0.162 |
| Rwork | 0.167 |
| R-free | 0.22100 |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 2.367 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.350 |
| Total number of observations | 244026 * | |
| Number of reflections | 79453 | |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 95.2 | 92.3 |
| Redundancy | 3.07 | 2.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.5 * | 298 | MES BUFFER, pH 6.5, MICRODIALYSIS, temperature 298K |
| 1 | MICRODIALYSIS | 7.5 * | 298 | MES BUFFER, pH 6.5, MICRODIALYSIS, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 1-2 (mg/ml) | |
| 2 | 1 | 1 | HEPES | 20 (mM) | |
| 3 | 1 | 1 | 150 (mM) | ||
| 4 | 1 | 2 | MES | 20 (mM) |
