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1DIZ

CRYSTAL STRUCTURE OF E. COLI 3-METHYLADENINE DNA GLYCOSYLASE (ALKA) COMPLEXED WITH DNA

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X25
Synchrotron siteNSLS
BeamlineX25
Temperature [K]138
Detector technologyCCD
Collection date1999-06-19
DetectorBRANDEIS
Spacegroup nameP 31 2 1
Unit cell lengths82.400, 82.400, 199.700
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution10.000 - 2.500
R-factor0.25
Rwork0.250
R-free0.29000
RMSD bond length0.010
RMSD bond angle21.700

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.590
High resolution limit [Å]2.4002.500
Rmerge0.0850.210
Number of reflections27877
<I/σ(I)>10
Completeness [%]98.0100
Redundancy88
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.529815% PEG 4000, 100MM HEPES, 100MM NACL, 50MM MGCL2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111HEPES
211NACL
311MGCL
411PEG 4000
512PEG 4000
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein8 (mg/ml)
21reservoirPEG400015 (%)
31reservoirHEPES100 (mM)
41reservoir100 (mM)
51reservoir50 (mM)
61reservoirehthylene glycol8 (%)

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PDB entries from 2024-11-06

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