1DIR

CRYSTAL STRUCTURE OF A MONOCLINIC FORM OF DIHYDROPTERIDINE REDUCTASE FROM RAT LIVER

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	222.200, 46.500, 94.300
Unit cell angles	90.00, 101.10, 90.00

Refinement procedure

Resolution	8.000 - 2.600
R-factor	0.167 *
Rwork	0.168
RMSD bond length	0.018
RMSD bond angle	3.500
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	5.130 *
High resolution limit [Å]	2.600 *	2.600 *
Rmerge	0.051 *
Total number of observations	26789 *
Completeness [%]		54.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8 *	277 *	referred to 1dhr *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4500	17 (%(w/v))
2	1	reservoir	Tris-HCl	0.05 (M)
3	1	reservoir	ethanol	11 (%(v/v))
4	1	drop	protein NADH solution	10 (mg/ml)	0.005ml
5	1	drop	PEG4500	17 (%(w/v))
6	1	drop	ethanol	11 (%(v/v))
7	1	drop	Tris-HCl	0.05 (M)