1DIF
HIV-1 PROTEASE IN COMPLEX WITH A DIFLUOROKETONE CONTAINING INHIBITOR A79285
Experimental procedure
Detector technology | IMAGE PLATE |
Detector | RIGAKU |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.000, 59.900, 62.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.700 |
R-factor | 0.198 |
Rwork | 0.198 |
RMSD bond length | 0.017 |
RMSD bond angle | 29.100 * |
Data reduction software | MOSFLM |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.700 * |
Rmerge | 0.092 * |
Total number of observations | 40709 * |
Number of reflections | 20479 |
Completeness [%] | 94.9 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.2 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protease | 8 (mg/ml) | |
2 | 1 | drop | sodium acetate | 50 (mM) | |
3 | 1 | drop | dithiothreitol | 10 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 37 (%(w/v)) | |
5 | 1 | reservoir | citrate | 63 (mM) | |
6 | 1 | reservoir | sodium phosphate | 126 (mM) | |
7 | 1 | reservoir | beta-mercaptoethanol | 1 (M) | |
8 | 1 | reservoir | dimethyl sulfoxide | ||
9 | 1 | reservoir | isopropanol |