1DHY

KKS102 BPHC ENZYME

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	IMAGE PLATE
Collection date	1994-06-11
Detector	MARRESEARCH
Spacegroup name	I 4 2 2
Unit cell lengths	123.000, 123.000, 110.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800 *
R-factor	0.204 *
Rwork	0.186
R-free	0.24300 *
RMSD bond length	0.011 *
RMSD bond angle	1.910 *
Data reduction software	WEIS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.076 *
Total number of observations	200071 *
Number of reflections	34668 *
Completeness [%]	88.4 *
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	12 *	Sugiyama, K., (1995) Proteins: Struct. Funct. Genet., 22, 284. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	17 (mg/ml)
2	1	reservoir	ammonium sulfate	0.9 (M)
3	1	reservoir	MPD	10 (%)
4	1	reservoir	Tris-HCl	100 (mM)	pH7.5