1DHP
DIHYDRODIPICOLINATE SYNTHASE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 122.410, 122.410, 111.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.662 |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Rmerge | 0.038 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 4 * | Laber, B., (1992) Biochem.J., 288, 691. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ammonium salfate | 1.25-3.0 (M) | |
| 2 | 1 | drop | sodium potassium phosphate | 1.25-3.0 (M) | |
| 3 | 1 | drop | sodium citrate | 0.8-1.6 (M) | |
| 4 | 1 | drop | phosphate | 0.2-1.5 (M) | |
| 5 | 1 | reservoir | PEG6000 | 10 (%(w/v)) |
