1DGM
CRYSTAL STRUCTURE OF ADENOSINE KINASE FROM TOXOPLASMA GONDII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-24 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.550, 68.750, 57.290 |
| Unit cell angles | 90.00, 100.30, 90.00 |
Refinement procedure
| Resolution | 46.780 - 1.800 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HUMAN ADENOSINE KINASE (PDB 1BX4) |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.043 | 0.210 * |
| Total number of observations | 317522 * | |
| Number of reflections | 33069 * | |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 98.6 | 89.3 |
| Redundancy | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | Recacha, R., (2000) Acta Crystallogr. Sect., D56, 76. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | reservoir | sodium sulfate | 1.0 (M) | |
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
| 4 | 1 | drop | adenosine | 0.020 (mM) | |
| 5 | 1 | drop | 2 (mM) |
