1DGB

HUMAN ERYTHROCYTE CATALASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	1998-03-23
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	83.497, 139.934, 227.891
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.176
Rwork	0.172
R-free	0.22700
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.075 *	0.131 *
Total number of observations	296455 *
Number of reflections	123184
<I/σ(I)>	14	4.2
Completeness [%]	90.7	82.8
Redundancy	2.4	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	6.5 - 8.0% PEG4000, protein at 40 mg/mL in 50 mM TrisCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	reservoir	PEG4000	6.5-8.0 (%)