1DFV
CRYSTAL STRUCTURE OF HUMAN NEUTROPHIL GELATINASE ASSOCIATED LIPOCALIN MONOMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-08-09 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 115.021, 115.021, 117.638 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.281 |
| Rwork | 0.281 |
| R-free | 0.29300 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 28.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.520 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.052 | 0.255 |
| Number of reflections | 25147 | |
| <I/σ(I)> | 43.8 | |
| Completeness [%] | 87.9 | 60 |
| Redundancy | 4.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 18 * | PEG 8k, Ammonium Sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 21-23 (%(w/w)) | |
| 3 | 1 | reservoir | ammonium sulfate | 12-14 (%sat) | |
| 4 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
| 5 | 1 | reservoir | PIPES | 25 (mM) | |
| 6 | 1 | reservoir | dithiothreitol | 10 (mM) |
