1DFN

CRYSTAL STRUCTURE OF DEFENSIN HNP-3, AN AMPHIPHILIC DIMER: MECHANISMS OF MEMBRANE PERMEABILIZATION

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	30.800, 45.000, 40.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.19
RMSD bond length	0.019
RMSD bond angle	0.049
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Total number of observations	22975 *
Number of reflections	4524 *
Completeness [%]	96.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	acetic acid	0.01 (%)
3	1	reservoir	PEG8000	15 (%)
4	1	reservoir	isopropanol	10 (%)
5	1	reservoir	sodium citrate	100 (mM)