1DFM
Crystal structure of restriction endonuclease BGLII complexed with DNA 16-mer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-28 |
| Detector | BRANDEIS - B4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.700, 101.800, 116.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.22400 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.078 | 0.289 |
| Total number of observations | 346887 * | |
| Number of reflections | 91032 | |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 96.6 | 97.5 |
| Redundancy | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 5.2 | 293 | 15- 20% PEG 4000, 0.2 M (NH4)2SO4, 0.1 M MES PH 5.2. CRYSTALS WERE LATER SOAKED WITH 5MM CACL2., VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | PEG 4000 | ||
| 3 | 1 | 1 | (NH4)2SO4 | ||
| 4 | 1 | 2 | PEG 4000 | ||
| 5 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | protein | 8-10 (mg/ml) | ||
| 2 | 1 | PEG1540 | 17-20 (%) | ||
| 3 | 1 | ammonium sulfate | 0.2 (M) | ||
| 4 | 1 | 1 | MES | 0.1 (M) |
