1DFK
NUCLEOTIDE-FREE SCALLOP MYOSIN S1-NEAR RIGOR STATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.500, 52.100, 164.600 |
| Unit cell angles | 90.00, 100.30, 90.00 |
Refinement procedure
| Resolution | 20.000 - 4.200 |
| R-factor | 0.415 |
| Rwork | 0.415 |
| R-free | 0.41000 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.400 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.270 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rmerge | 0.063 | 0.308 |
| Total number of observations | 139608 * | |
| Number of reflections | 10676 * | |
| <I/σ(I)> | 27 | |
| Completeness [%] | 97.9 | 85.2 |
| Redundancy | 1.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 277 | drop consists of equal amounts of protein and reservoir solutions * |
| 1 | Vapor diffusion, sitting drop * | 7 | 277 | drop consists of equal amounts of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 7 (%(v/v)) | |
| 3 | 1 | reservoir | MES | 50 (mM) | |
| 4 | 1 | reservoir | 3 (mM) | ||
| 5 | 1 | drop | MgADP/VO4 | 0.5 (mM) |
