1DFF

PEPTIDE DEFORMYLASE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-08
Detector	RIGAKU
Spacegroup name	P 61 2 2
Unit cell lengths	55.350, 55.350, 230.920
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.880
R-factor	0.184
Rwork	0.184
R-free	0.28900
RMSD bond length	0.013
RMSD bond angle	24.384 *
Data reduction software	R-AXIS
Data scaling software	R-AXIS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.880
Rmerge	0.091 *
Total number of observations	73018 *
Number of reflections	4903
Completeness [%]	91.6	80.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	sodium phosphate	20 (mM)
3	1	drop		10 (mM)
4	1	drop	ammonium sulfate	1.0 (M)
5	1	reservoir	sodium acetate	0.1 (M)
6	1	reservoir	sodium formate	2.0 (M)