1DFB

STRUCTURE OF A HUMAN MONOCLONAL ANTIBODY FAB FRAGMENT AGAINST GP41 OF HUMAN IMMUNODEFICIENCY VIRUS TYPE I

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	66.600, 74.700, 105.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 2.700
R-factor	0.177
Rwork	0.177
RMSD bond length	0.020
RMSD bond angle	4.000
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.700 *
Total number of observations	77314 *
Number of reflections	13529 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		22 *	referred to J.Mol.Biol. 216.511-512 1990 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	25-30 (%(w/v))
2	1	reservoir	sodium phosphate	0.05 (M)
3	1	drop	Fab	10 (mg/ml)
4	1	drop	PEG3350	20-35 (%)
5	1	drop	sodium phosphate	0.05 (M)