1DF8

S45A MUTANT OF STREPTAVIDIN IN COMPLEX WITH BIOTIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	127
Detector technology	IMAGE PLATE
Collection date	1998-10-23
Detector	RIGAKU
Spacegroup name	I 2 2 2
Unit cell lengths	46.200, 93.900, 104.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.510
R-factor	0.165 *
Rwork	0.169
R-free	0.21700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1swa
RMSD bond length	0.009 *
RMSD bond angle	0.029 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.052	0.103
Total number of observations	167399 *
Number of reflections	30427
<I/σ(I)>	2.15
Completeness [%]	82.4	4
Redundancy	5.5	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.5	294	40% SATURATED AMMONIUM SULFATE, 0.1 M SODIUM ACETATE, pH 4.5, temperature 294.K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	40 (%sat)
2	1	1	sodium acetate	0.1 (M)
3	1	1	biotin	3.8 (mM)