1DET
RIBONUCLEASE T1 CARBOXYMETHYLATED AT GLU 58 IN COMPLEX WITH 2'GMP
Experimental procedure
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 288 |
Detector technology | IMAGE PLATE |
Collection date | 1995-05-16 |
Detector | RIGAKU |
Spacegroup name | I 2 3 |
Unit cell lengths | 88.690, 88.690, 88.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.800 |
R-factor | 0.194 * |
RMSD bond length | 0.019 |
RMSD bond angle | 0.045 |
Data reduction software | WEIS |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 1.800 | 1.800 * |
Rmerge | 0.057 | |
Number of reflections | 10459 | |
Completeness [%] | 95.9 | 89.7 * |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 3.2 * | 20 * | pH 4.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | 2'-GMP | 0.5 (%) | |
3 | 1 | drop | sodium citrate | 30 (mM) | |
4 | 1 | drop | 2-methylpentane-2,4-diol | 17 (%(v/v)) | |
5 | 1 | reservoir | 2-methylpentane-2,4-diol | 51 (%(v/v)) |