1DEQ
THE CRYSTAL STRUCTURE OF MODIFIED BOVINE FIBRINOGEN (AT ~4 ANGSTROM RESOLUTION)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-10-21 |
| Detector | BRANDEIS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 176.006, 94.935, 209.805 |
| Unit cell angles | 90.00, 94.41, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.500 |
| R-factor | 0.263 |
| Rwork | 0.257 |
| R-free | 0.37000 |
| Structure solution method | molecular replacement (using human fragment d coordinates) |
| Starting model (for MR) | human fragment d coordinates (see related entry 1fza) |
| RMSD bond length | 0.012 |
| RMSD bond angle | 28.100 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 210.000 | 3.570 |
| High resolution limit [Å] | 3.400 | 3.380 |
| Rmerge | 0.075 | 0.280 |
| Number of reflections | 79465 | |
| <I/σ(I)> | 7.05 | |
| Completeness [%] | 78.5 | 46.7 |
| Redundancy | 4.42 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.2 | 277 | 10 mM MES, 5mM sodium azide, 2mM calcium chloride , pH 6.2, temperature 277K |
| 1 | Batch method * | 6.2 | 277 | 10 mM MES, 5mM sodium azide, 2mM calcium chloride , pH 6.2, temperature 277K |
| 1 | Batch method * | 6.2 | 277 | 10 mM MES, 5mM sodium azide, 2mM calcium chloride , pH 6.2, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 2.6 (mg/ml) | |
| 2 | 1 | 1 | MES | 10 (mM) | |
| 3 | 1 | 1 | 5 (mM) | ||
| 4 | 1 | 1 | 2 (mM) |
