1DEJ

CRYSTAL STRUCTURE OF A DICTYOSTELIUM/TETRAHYMENA CHIMERA ACTIN (MUTANT 646: Q228K/T229A/A230Y/A231K/S232E/E360H) IN COMPLEX WITH HUMAN GELSOLIN SEGMENT 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-06-29
Detector	OTHER
Spacegroup name	P 21 21 21
Unit cell lengths	56.893, 69.385, 183.241
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.171 *
Rwork	0.171
R-free	0.21400
RMSD bond length	0.013 *
RMSD bond angle	1.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.033	0.055
Total number of observations	96715 *
Number of reflections	27352 *
<I/σ(I)>	29.6
Completeness [%]	93.5	88.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	293	MES, NACL, CACL2, MGCL2, ATP, PEG6000, DTT, NAN3, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
10	1	reservoir		150 (mM)
11	1	reservoir	ATP	0.1 (mM)
12	1	reservoir		0.1 (mM)
13	1	reservoir		0.1 (mM)
14	1	reservoir		1 (mM)
15	1	reservoir	dithiothreitol	10 (mM)
2	1	drop	imidazole-HCl	10 (mM)
3	1	drop		150 (mM)
4	1	drop	ATP	0.1 (mM)
5	1	drop		0.1 (mM)
6	1	drop		0.1 (mM)
7	1	drop		1 (mM)
8	1	drop	dithiothreitol	0.1 (mM)
9	1	reservoir	imidazole-HCl	50 (mM)