1DEJ
CRYSTAL STRUCTURE OF A DICTYOSTELIUM/TETRAHYMENA CHIMERA ACTIN (MUTANT 646: Q228K/T229A/A230Y/A231K/S232E/E360H) IN COMPLEX WITH HUMAN GELSOLIN SEGMENT 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6B |
Synchrotron site | Photon Factory |
Beamline | BL-6B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-06-29 |
Detector | OTHER |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.893, 69.385, 183.241 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.400 |
R-factor | 0.171 * |
Rwork | 0.171 |
R-free | 0.21400 |
RMSD bond length | 0.013 * |
RMSD bond angle | 1.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.033 | 0.055 |
Total number of observations | 96715 * | |
Number of reflections | 27352 * | |
<I/σ(I)> | 29.6 | |
Completeness [%] | 93.5 | 88.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 293 | MES, NACL, CACL2, MGCL2, ATP, PEG6000, DTT, NAN3, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
10 | 1 | reservoir | 150 (mM) | ||
11 | 1 | reservoir | ATP | 0.1 (mM) | |
12 | 1 | reservoir | 0.1 (mM) | ||
13 | 1 | reservoir | 0.1 (mM) | ||
14 | 1 | reservoir | 1 (mM) | ||
15 | 1 | reservoir | dithiothreitol | 10 (mM) | |
2 | 1 | drop | imidazole-HCl | 10 (mM) | |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | drop | ATP | 0.1 (mM) | |
5 | 1 | drop | 0.1 (mM) | ||
6 | 1 | drop | 0.1 (mM) | ||
7 | 1 | drop | 1 (mM) | ||
8 | 1 | drop | dithiothreitol | 0.1 (mM) | |
9 | 1 | reservoir | imidazole-HCl | 50 (mM) |