1DEB

CRYSTAL STRUCTURE OF THE N-TERMINAL COILED COIL DOMAIN FROM APC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	76
Detector technology	IMAGE PLATE
Collection date	1994
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 65 2 2
Unit cell lengths	66.860, 66.860, 127.470
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.2326 *
Rwork	0.234
R-free	0.27460 *
RMSD bond length	0.015
RMSD bond angle	1.570
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.480
High resolution limit [Å]	2.400	2.400
Rmerge	0.078 *	0.176 *
Total number of observations	18321 *
Number of reflections	6743 *
<I/σ(I)>	5.3
Completeness [%]	96.4 *	96.7 *
Redundancy	2.8	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.8	277	15% PEG 4000, 0.1M AMMONIUM ACETATE, 0.1M NA ACETATE, pH 4.8, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	peptide	20 (mg/ml)
2	1	reservoir	PEG4000	15 (%(w/v))
3	1	reservoir	ammonium sulfate	0.1 (M)
4	1	reservoir	sodium acetate	0.1 (M)