1DEA

STRUCTURE AND CATALYTIC MECHANISM OF GLUCOSAMINE 6-PHOSPHATE DEAMINASE FROM ESCHERICHIA COLI AT 2.1 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	H 3 2
Unit cell lengths	125.710, 125.710, 222.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.100
R-factor	0.174
Rwork	0.174
R-free	0.22400 *
RMSD bond length	0.006
RMSD bond angle	23.700 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.100 *
Rmerge	0.101 *
Number of reflections	38368 *
Completeness [%]	97.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium potassium phosphate	1.33 (M)