1DE6

L-RHAMNOSE ISOMERASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	195
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Spacegroup name	C 1 2 1
Unit cell lengths	169.179, 162.439, 77.726
Unit cell angles	90.00, 109.89, 90.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.173
Rwork	0.244
R-free	0.24400
RMSD bond length	0.019 *
RMSD bond angle	2.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.134	0.426
Number of reflections	108398
<I/σ(I)>	6.8
Completeness [%]	92.0	88 *
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	4 *	0.2 M CITRATE, 0.1-M HEPES, 22.5 - 30.0 % PEG 8000, 10 % ISOPROPANOL, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	drop		100 (mM)
4	1	reservoir	citrate	0.2 (M)
5	1	reservoir	HEPES	0.1 (M)
6	1	reservoir	PEG8000	22.5-30.0 (%(w/w))
7	1	reservoir	isopropanol	10 (%(w/w))