1DE4

HEMOCHROMATOSIS PROTEIN HFE COMPLEXED WITH TRANSFERRIN RECEPTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-01-07
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	110.400, 144.400, 327.100
Unit cell angles	90.00, 93.60, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.231
Rwork	0.231
R-free	0.26500
Structure solution method	MIRAS
RMSD bond length	0.007
RMSD bond angle	23.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.070	0.341
Number of reflections	122846
<I/σ(I)>	18	3.3
Completeness [%]	98.1	99.8
Redundancy	2.8	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 8000, TRIS, TRIMETHYLAMINE HCL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	7-8 (%)
2	1	reservoir	Tris-HCl	200 (mM)
3	1	reservoir	trimethylamine HCl	10 (mM)