1DDZ
X-RAY STRUCTURE OF A BETA-CARBONIC ANHYDRASE FROM THE RED ALGA, PORPHYRIDIUM PURPUREUM R-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.500, 112.800, 73.200 |
| Unit cell angles | 90.00, 103.60, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.27400 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.071 | 0.227 |
| Total number of observations | 326527 * | |
| Number of reflections | 50489 | 5016 * |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 20 * | PEG4000, AMMONIUM SULFATE, SODIUM CACODYLATE, pH 6.75, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 24 (%) | |
| 5 | 1 | reservoir | ammonium sulfate | 300 (mM) | |
| 6 | 1 | reservoir | sodium cacodylate | 50 (mM) |
