1DDW

HOMER EVH1 DOMAIN UNLIGANDED

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	1998-10-30
Detector	RIGAKU RAXIS IV
Spacegroup name	P 32 2 1
Unit cell lengths	49.940, 49.940, 80.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.245
Rwork	0.245
R-free	0.28500
RMSD bond length	0.013
RMSD bond angle	1.745
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.780
High resolution limit [Å]	1.700	1.700
Rmerge	0.081 *	0.286
Number of reflections	24051 *
<I/σ(I)>	21
Completeness [%]	96.8 *	77
Redundancy	4.8	2.34

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3 *	293	PEG 3350, Magnesium sulfate, Hepes, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.3 *	293	PEG 3350, Magnesium sulfate, Hepes, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.3 *	293	PEG 3350, Magnesium sulfate, Hepes, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.3 *	293	PEG 3350, Magnesium sulfate, Hepes, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	reservoir	PEG3350	30 (%)
3	1	reservoir		87 (mM)
4	1	reservoir	HEPES	50 (mM)