1DDO

REDUCED D-AMINO ACID OXIDASE FROM PIG KIDNEY IN COMPLEX WITH IMINO-TRP

Experimental procedure

Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1996-01
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	325.200, 137.100, 196.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.100
R-factor	0.236
Rwork	0.236
R-free	0.25000
Structure solution method	DENSITY AVERAGING
Starting model (for MR)	1kif
RMSD bond length	0.023
RMSD bond angle	3.029
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	DM
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.260
High resolution limit [Å]	3.100	3.100
Rmerge	0.093	0.236
Total number of observations	514100 *
Number of reflections	77977
<I/σ(I)>	5	3
Completeness [%]	98.9	98.7
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.3		PROTEIN WAS CRYSTALLIZED FROM 0.5 M AMMONIUM SUCCINATE, 100 MM TRIS PH 8.3, 2MM BENZOATE THEN SOAKED IN 20MM D-TRP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium succinate	0.6 (M)