1DDI
CRYSTAL STRUCTURE OF SIR-FP60
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 98.610, 98.610, 123.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.510 |
| Rwork | 0.185 |
| R-free | 0.24500 |
| Structure solution method | MIR |
| RMSD bond length | 0.021 * |
| RMSD bond angle | 0.036 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.510 |
| Rmerge | 0.099 |
| Total number of observations | 212439 * |
| Number of reflections | 23679 |
| <I/σ(I)> | 6.2 |
| Completeness [%] | 98.4 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 277 | AMMONIUM SULFATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 4K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | NADP+ | 2.5 (mM) | |
| 3 | 1 | reservoir | Tris-HCl | 50 (mM) | pH7.0 |
| 4 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 5 | 1 | reservoir | MPD | 10 (%(w/v)) |
