1DDG
CRYSTAL STRUCTURE OF SIR-FP60
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 34.840, 82.540, 105.360 |
Unit cell angles | 79.71, 83.05, 89.90 |
Refinement procedure
Resolution | 10.000 - 2.010 |
R-free | 0.28900 |
Structure solution method | MOL. REPL. |
Starting model (for MR) | SIR60-FL |
RMSD bond length | 0.012 * |
RMSD bond angle | 0.024 * |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.010 |
Rmerge | 0.083 |
Total number of observations | 304582 * |
Number of reflections | 76269 * |
<I/σ(I)> | 4.5 |
Completeness [%] | 95.9 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 277 | AMMONIUM SULFATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 4K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.5 (mg/ml) | |
2 | 1 | drop | NADP+ | 2.5 (mM) | |
3 | 1 | reservoir | Tris-HCl | 50 (mM) | pH7.0 |
4 | 1 | reservoir | ammonium sulfate | 2 (M) | |
5 | 1 | reservoir | MPD | 10 (%(w/v)) |