1DD9
STRUCTURE OF THE DNAG CATALYTIC CORE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1999-06-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.340, 57.870, 148.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| Rwork | 0.231 |
| R-free | 0.27600 |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.036 | 0.177 |
| Total number of observations | 182728 * | |
| Number of reflections | 43387 | |
| <I/σ(I)> | 27.2 | |
| Completeness [%] | 97.2 | 94.9 |
| Redundancy | 4.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | 18-21% PEG4000, 5% PEG200, 30% ETHYLENE GLYCOL, 0.2M AMMONIUM ACETATE, 0.05M SODIUM ACETATE, PH 5.0, 0.1% DIOXANE, 2-8 MM SRCL2, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | |
| 10 | 1 | reservoir | 2-8 (mM) | or YCl2, DyCl3 | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | reservoir | PEG4000 | 18-21 (%) | |
| 5 | 1 | reservoir | PEG200 | 5 (%) | |
| 6 | 1 | reservoir | ethylene glycol | 30 (%) | |
| 7 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 8 | 1 | reservoir | sodium acetate | 0.05 (M) | |
| 9 | 1 | reservoir | dioxane | 0.1 (%) |
