1DD8
CRYSTAL STRUCTURE OF BETA-KETOACYL-[ACYL CARRIER PROTEIN] SYNTHASE I FROM ESCHERICHIA COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1996-06-15 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.620, 142.710, 213.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.300 |
Rwork | 0.188 |
R-free | 0.21900 |
RMSD bond length | 0.013 * |
RMSD bond angle | 1.540 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.071 | 0.119 |
Number of reflections | 76863 | |
Completeness [%] | 94.3 | 51 |
Redundancy | 3.24 | 1.44 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | Olsen, J.G., (1995) Protein Pept. Lett., 1, 246. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
2 | 1 | reservoir | PEG400 | 3 (%) | |
3 | 1 | reservoir | HEPES-NaOH | 0.1 (M) |