1DD5

CRYSTAL STRUCTURE OF THERMOTOGA MARITIMA RIBOSOME RECYCLING FACTOR, RRF

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-03-27
Detector	MARRESEARCH
Wavelength(s)	0.9786,0.9788,0.9184
Spacegroup name	P 43 21 2
Unit cell lengths	47.250, 47.250, 297.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.550
R-factor	0.232
Rwork	0.230
R-free	0.27700
RMSD bond length	0.006
RMSD bond angle	22.300 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.650
High resolution limit [Å]	2.550	2.550
Rmerge	0.049	0.153
Number of reflections	11648
<I/σ(I)>	27	9.5
Completeness [%]	97.4	91.8
Redundancy	6.7	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6 *	298	drop consists of equal volume of protein and reservoir solutions *
1	Vapor diffusion, hanging drop *	7.6 *	298	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop		50 (mM)
4	1	drop	dithiothreitol	0.5 (mM)
5	1	reservoir	sodium acetate	0.1 (M)
6	1	reservoir	ammonium sulfate	2.0 (M)