1DD4

Crystal structure of ribosomal protein l12 from thermotoga maritim

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	I 21 3
Unit cell lengths	144.744, 144.744, 144.744
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.229
Rwork	0.229
R-free	0.24100
RMSD bond length	0.008
RMSD bond angle	19.360 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.051	0.166
Number of reflections	19877
<I/σ(I)>	12.5
Completeness [%]	91.2	93.7
Redundancy	6.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *	291	3.23M AMMONIUM SULFATE, 0.1M CITRATE, 7% (V/V) POLYETHYLENE GLYCOL 200, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11 (mg/ml)
2	1	drop	HEPES	7.1 (mM)
3	1	drop	ammonium sulfate	0.692 (M)
4	1	drop	sodium citrate	0.02 (M)
5	1	drop	PEG200	2.1 (%)
6	1	reservoir	ammonium sulfate	3.23 (M)
7	1	reservoir	sodium citrate	0.1 (M)