1DD3
CRYSTAL STRUCTURE OF RIBOSOMAL PROTEIN L12 FROM THERMOTOGA MARITIMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.330, 89.130, 119.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.23500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 19.710 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.078 | 0.367 * |
| Number of reflections | 20073 | |
| <I/σ(I)> | 22.1 | |
| Completeness [%] | 98.6 | 95.1 * |
| Redundancy | 3.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 291 | 3.6M AMMOMIUM SULFATE 0.1M SODIUM CITRATE 7% (V/V) POLYETHYLENE GLYCOL 200, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | drop | HEPES | 7.1 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 0.692 (M) | |
| 4 | 1 | drop | sodium citrate | 0.02 (M) | |
| 5 | 1 | drop | PEG200 | 2.1 (%) | |
| 6 | 1 | reservoir | ammonium sulfate | 3.23 (M) | |
| 7 | 1 | reservoir | sodium citrate | 0.1 (M) |
