1DCU
REDOX SIGNALING IN THE CHLOROPLAST: STRUCTURE OF OXIDIZED PEA FRUCTOSE-1,6-BISPHOSPHATE PHOSPHATASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE D41A |
| Synchrotron site | LURE |
| Beamline | D41A |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.604, 126.316, 78.023 |
| Unit cell angles | 90.00, 97.73, 90.00 |
Refinement procedure
| Resolution | 23.800 - 2.200 |
| Rwork | 0.189 |
| R-free | 0.24700 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.900 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.068 | 0.270 |
| Total number of observations | 344547 * | |
| Number of reflections | 69625 | |
| Completeness [%] | 99.4 | 96.8 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | PEG 400, Na acetate, magnesium chloride, fructose-6-phosphate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 25 (%) | |
| 2 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 3 | 1 | reservoir | 50 (mM) | ||
| 4 | 1 | reservoir | F6P | 5 (mM) | |
| 5 | 1 | reservoir | TCEP | 5 (mM) |
