1DCR
CRYSTAL STRUCTURE OF DNA SHEARED TANDEM G-A BASE PAIRS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1996-06-17 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 20.950, 61.860, 68.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
R-factor | 0.192 * |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB STRUCTURE 1D9R |
RMSD bond length | 0.009 |
RMSD bond angle | 0.025 |
Data scaling software | d*TREK |
Phasing software | X-PLOR |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.075 | 0.445 |
Number of reflections | 5771 | |
<I/σ(I)> | 19.2 | 3.8 |
Completeness [%] | 85.1 * | 93.7 |
Redundancy | 2.61 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 298 | MPD, MGCL2, TRIS, SPERMINE, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | SPERMINE | ||
2 | 1 | 1 | MGCL2 | ||
3 | 1 | 1 | TRIS | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA duplex | 1.3 (mM) | |
2 | 1 | drop | Tris-HCl | 2 (mM) | |
3 | 1 | drop | 5 (mM) | ||
5 | 1 | drop | 2.6 (%) | ||
6 | 1 | reservoir | MPD | 40 (%) |