1DCN
INACTIVE MUTANT H162N OF DELTA 2 CRYSTALLIN WITH BOUND ARGININOSUCCINATE
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 120 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.580, 99.590, 107.140 |
Unit cell angles | 90.00, 101.68, 90.00 |
Refinement procedure
Resolution | 17.000 * - 2.300 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1auw |
RMSD bond length | 0.006 * |
RMSD bond angle | 18.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE (5.3) |
Refinement software | CNS (0.3C) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.079 * | 0.233 |
Total number of observations | 194258 * | |
Number of reflections | 77291 | |
Completeness [%] | 90.3 | 96.4 * |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 | pH 8.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme solution | 14 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 100 (mM) | |
3 | 1 | reservoir | PEG MME2000 | 18 (%(w/v)) | |
4 | 1 | reservoir | 200 (mM) | ||
5 | 1 | reservoir | Tris-HCl | 100 (mM) |